Human Thrombin (TM) ELISA Kit

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This kit is only for scientific research, and shall not be used as a clinical diagnosis of use.

Purpose

This kit allows for the determination of TM concentrations in Human serum, cell culture supernatant, and other biological fluids.

Principle The kit assay Human TM level in the sample，add Human TM antibody to microtiter plate wells, after Incubating,add Biotinylated anti –TM -antibody , then Combined Streptavidin-HRP, become complex, after Incubating and washing Completely, Add TMB substrate solution,TMB substrate becomes blue color, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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